Core Facilities with Seahorse XF Research Servicesīasic procedures for seeding adherent cells (typically performed the day before the XF p HS Mini assay)įor each density to be tested, seed as directed for adherent cells.Agilent Cell Analysis Publication Databaseīrowse the full list of publications using Agilent Cell Analysis data.Agilent Seahorse Assay Guides and Templates.Please consult the Agilent Cell Analysis Publication Database and/or the XF Publication database to provide an initial starting point for cell density values specific to your needs. Generally, densities resulting in 50-90% confluency generate metabolic rates in the desirable/dynamic range of the instrument. Suspension cells require higher seeding density from 5 x 10 4 to 2 x 10 5 cells per well depending on cell type. Optimal cell seeding number varies by cell type, but is typically between 5 x 10 3 and 4 x 10 4 cells per well for adherent cells. The Seahorse XF Real-Time ATP rate assay Test Kit The Seahorse XFp Real-Time ATP rate assay Test Kit can be used to characterize the cell line/type of interest in a single assay. To effectively examine metabolic and bioenergetic function using your Agilent Seahorse Extracellular Flux Analyzer, Analyzer, it is essential to first characterize a specific cell type with respect to its metabolic activity under basal and maximal respiration (OCR) and extracellular acidification (ECAR). Support Services, Agreements & Trainingīasic procedures for choosing cell seeding densities.Shorten the time it takes to start seeing the full value of your instrument investment
Instrument & equipment deinstallation, transportation, and reinstallationĬrossLab Connect services use laboratory data to improve control and decision-makingĪdvance lab operations with lab-wide services, asset management, relocation Learn essential lab skills and enhance your workflows Instrument/software qualifications, consulting, and data integrity validations Software to manage instrument access/usage, sample processing, inventories, and more Service Plans, On Demand Repair, Preventive Maintenance, and Service Center Repair
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